Membrane-24h-15 - SRA

SRX3961879: Membrane-24h-15
1 ILLUMINA (Illumina MiSeq) run: 42,351 spots, 22.2M bases, 13.8Mb downloads

Design: Amplification of the 16S V6-V8 region (16S rDNA) was performed using a two-step dual-indexed PCR approach specifically designed for Illumina instruments. In a first step, the gene specific sequence is fused to the Illumina TruSeq sequencing primers and PCR was carried out. The second PCR step, performed with 50- to 100-fold dilution of the previously purified PCR product as a DNA template, allowed the addition of barcodes (dual-indexed) and the missing sequence required for Illumina sequencing. Barcoded amplicons were then pooled in equimolar concentration for sequencing on the Illumina Miseq.

Submitted by: Laval University

Study: Dairy biofilm formation on filtration membrane used in a bioreactor Raw sequence reads

PRJNA450825 • SRP140914 • All experiments • All runs

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The goal of this study was to analyse the biofilm formation on membranes as a function of the processing time at various operation temperature (15 vs 50°C).

Sample: Bacteria on membrane after 24h at 15°C

SAMN08948199 • SRS3192024 • All experiments • All runs

Organism: biofilter metagenome

Library:

Name: 22-3-Mem-24h-15

Instrument: Illumina MiSeq

Strategy: AMPLICON

Source: METAGENOMIC

Selection: PCR

Layout: PAIRED

Runs: 1 run, 42,351 spots, 22.2M bases, 13.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR7029615	42,351	22.2M	13.8Mb	2019-05-01

ID:: 5428463

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